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Note.The apparent molecular weight (kDa) of each protein has been determined bycalibration against an unstained protein standard; supplemental data should beconsidered for more accurate adjustments in different electrophoresisconditions.
LoadingVolume
l 3μl or 5 μl per loading for clear visualization during electrophoresis on a15-well or 10-well mini-gel.
l 1.5~2.5μl per well for general Western blotting transfer.
l Applymore for a thicker (> 1.5 mm) or larger gel.
Description
The 3-color High Range Protein Markeris a ready to use three-color protein standard with 12 pre-stained proteinscovering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES)buffer). Proteins are covalently coupled with different chromophores for easyidentification of bands, with two reference proteins carrying enhancedintensity corresponding to a green at25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).
The 3-color High Range Protein Markeris designed for monitoring protein separation during SDS-polyacrylamide gelelectrophoresis, verification of Western transfer efficiency on membranes(PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
The marker is supplied in gel loadingbuffer and is ready to use. Do NOT heat, dilute, or add reducing agentsbefore loading.
Contents
Approximately0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Guidefor Molecular Weight Estimation
Migration patternsand approximate MWs (kDa) of 3-color High Range Protein Marker in differentelectrophoresis conditions are listed below:
QualityControl
Undersuggested conditions, 3-color High Range Protein Marker resolves 12 major bandsin SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blottingtransfer to a nitrocellulose membrane.
Otherinformation
GENEFIST Life Science, Inc. claims all warranties withrespect to this document, expressed or implied, including but not limited tothose of merchantability or fitness for a particular purpose. In no event shallGENEFIST Life Science, Inc. be liable, whether in contract, tort, warranty, orunder any statute or any other basis for special, incidental, indirect,punitive, multiple or consequential damages in connection with or arising fromthis document, including but not limited to the use there of.
For research use only
Caution: Not intended for human or animal diagnosticor therapeutic uses.